In vitro screening of mutant medicago sativa genotypes for drought stress tolerance at germination stage
İskender TİRYAKİ, Uğur SARI, Okan Acar, Çağlar KAYA, Selçuk ÇETİN
Canakkale Onsekiz Mart University, Turkey
Drought is one of the major abiotic stress factors threatening agricultural production all around the world. Perennial legume plant alfalfa (Medicago sativa) is the most important forage crop of moderate climate region. Along with deep root structure, mature alfalfa is generally considered as drought tolerant. However, alfalfa is especially sensitive to drought at germination, seedling and right after cutting. Various studies provided that poly ethylene glycol (PEG) can successfully be used to provide drought stress at controlled conditions. The mutant M3 seeds were screened in a half strength root growth MS media containing 35% PEG under in vitro conditions to create drought stress environment in a root growth medium. Tolerant genotypes were determined in the screening and were transplanted to pots and then be grown in growth chamber. Candidate drought tolerant mutant genotypes were grazed at 5 cm cutting height when the first flowering buds visible and drought stress were applied for 24 days. Some morphological, physiological (Protein, MDA, APX and SOD enzyme activities) and molecular aspects (drought related gene expression levels) of drought response parameters were determined and were compared with control plants.
Audience Take Away:
Prof. Dr. Iskender Tiryaki is affiliated with Department of Agricultural Biotechnology, Faculty of Agriculture, Canakkale Onsekiz Mart University, Canakkale, Turkey. He received his M.Sc. and Ph.D. from Agronomy (Plant Breeding and Genetics) Department of University of Nebraska, Lincoln-NE, USA. His primary research area is Plant Breeding and Genetics, and Plant Molecular Genetics and Biotechnology. He is also interested in seed priming, seed germination and seedling emergence of crop plants in adverse conditions. He has more than 60 high research quality papers, published in Plant Cell, Plant Physiology, Agronomy Journal, Seed Science and Technology, Journal of Pineal Research, Plant Genetic Resources: Characterization and Utilization, etc. He has also authored of two the books entitled ‘Plant Breeding and Genetics’ and ‘Forward Genetics: Finding Mutants that Defect in Jasmonic Acid/Auxin Signaling Pathways in Arabidopsis’. He is serving as editorial board member or reviewer of several articles for reputed journals across the globe, including Nature Scientific Reports. He is serving as department head of Agricultural Biotechnology at Faculty of Agriculture, Canakkale Onsekiz Mart University, Turkey.