Comprehensive characterization by RP-UHPLC-ESI-Q-TOF-MS/MS from Ammoides atlantica extract and their antioxidant activities
Tarek BOUDIAR, Ph.D.1*, Chawki BENSOUICI, Ph.D.1, Jesús LOZANO-SÁNCHEZ, Ph.D.2, Antonio SEGURA-CARRETERO2
1Biotechnology Research Center (C.R.Bt – Constantine), Algeria
2Research and Development of Functional Food Centre (CIDAF) Granada, Spain
Two Ammoides (Apiaceae) species, A. pusella and A. atlantica are found in Algeria. These species are used in folk medicine as antibacterial, anti-diarrhoea, anti-fever, antiinfluenza and to treat vitiligo in addition of their use as condiments. The aqueous extract of A. pusilla is used in Eastern Morocco to treat diabetes. The healthy effect of these leaves could be attributed to their composition, especially, phenolic compounds.
Methanolic extract of Ammoides atlantica (Apiaceae) leaves and flowers were evaluated. Phytochemical composition was determined by reversed-phase high-performance liquid chromatography (RP-HPLC) coupled to quadruple-time-of-ﬂight mass spectrometry (QTOF-MS) using negative and positive ionization modes.
The analytical methodology applied enabled the characterization of 60 compounds. These compounds were distributed in two major categories namely hydroxycinnamic acid derivatives, and flavonoids.
In-vitro antioxidant activity was evaluated from methanol leaves and flowers extract using different antioxidant tests, namely, total antioxidant (DPPH) scavenging, (ABTS•+) scavenging, superoxide radical scavenging by alkaline DMSO, reducing power, cupric reducing antioxidant capacity (CUPRAC) and ferrous ions chelating assays compared to the synthetic antioxidants (BHA, BHT and Acid ascorbic).
Concerning DPPH (23.3 ± 0.9), ABTS•+ (IC50: 11 ± 2) and CUPRAC (14 ± 1) assays, hydro-alcoholic extract showed lower antioxidant activity compared to the substances of reference BHA (6.14 ± 0.41), BHT (12.9 ± 0.4) and ascorbic acid (14 ± 3). Nevertheless A. atlantica extract showed better antioxidant activity that the available standard for the O2- DMSO alkaline (3.2 ± 0.2) compared to BHT (>200) and BHA (>200).
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Tarek BOUDIAR has completed his Ph.D. degree at the age of 32 years from Mentouri University, Constantine (Algeria). He is deputy director of the Biotechnology Research Centre (C.R.Bt) Constantine. He has over 08 publications and his publication H-index is 05.